The four Republican presidential hopefuls will gather on a debate stage at the University of Miami on Thursday night for what's likely to be a dramatic high-stakes debate.
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It is schedule just five days before the crucial winner-take-all Florida and Ohio primaries, the home states of presidential candidates Marco Rubio and John Kasich.
The debate will be co-hosted by CNN/Salem Radio/ Washington Times.
Rubio has a lot riding on how well he performs; how much of a political punch-up will occur between Rubio, Ted Cruz and front-runner Donald Trump?
In an MSNBC townhall Wednesday, Rubio told NBC Moderator of Meet the Press Chuck Todd, he regrets some of the attacks he's levied on Trump.
Rubio: "My kids were embarrassed, and if I had to do it again, I wouldn't."
Todd: "It took a toll on (Trump)."
Rubio: "Well, I don't know about political toll."
Todd: "Well, what I'm saying, you didn't look comfortable doing it."
Rubio: "Well, look he needed to be stood up. This is a guy that's basically offended everyone. For a year, I mean, he has literally mocked a disabled journalist, a female journalist, every minority group imaginable on a daily basis."
Some supporters have said the attacks have worked against Rubio. We'll see if he continues them tonight during the debate, which starts at 8:30 p.m.