All 49 names are now listed on the city of Orlando's victims website. They are:
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- Stanley Almodovar III, 23 years old
- Amanda Alvear, 25 years old
- Oscar A Aracena-Montero, 26 years old
- Rodolfo Ayala-Ayala, 33 years old
- Antonio Davon Brown, 29 years old
- Darryl Roman Burt II, 29 years old
- Angel L. Candelario-Padro, 28 years old
- Juan Chevez-Martinez, 25 years old
- Luis Daniel Conde, 39 years old
- Cory James Connell, 21 years old
- Tevin Eugene Crosby, 25 years old
- Deonka Deidra Drayton, 32 years old
- Simon Adrian Carrillo Fernandez, 31 years old
- Leroy Valentin Fernandez, 25 years old
- Mercedez Marisol Flores, 26 years old
- Peter O. Gonzalez-Cruz, 22 years old
- Juan Ramon Guerrero, 22 years old
- Paul Terrell Henry, 41 years old
- Frank Hernandez, 27 years old
- Miguel Angel Honorato, 30 years old
- Javier Jorge-Reyes, 40 years old
- Jason Benjamin Josaphat, 19 years old
- Eddie Jamoldroy Justice, 30 years old
- Anthony Luis Laureanodisla, 25 years old
- Christopher Andrew Leinonen, 32 years old
- Alejandro Barrios Martinez, 21 years old
- Brenda Lee Marquez McCool, 49 years old
- Gilberto Ramon Silva Menendez, 25 years old
- Kimberly Morris, 37 years old
- Akyra Monet Murray, 18 years old
- Luis Omar Ocasio-Capo, 20 years old
- Geraldo A. Ortiz-Jimenez, 25 years old
- Eric Ivan Ortiz-Rivera, 36 years old
- Joel Rayon Paniagua, 32 years old
- Jean Carlos Mendez Perez, 35 years old
- Enrique L. Rios, Jr., 25 years old
- Jean C. Nives Rodriguez, 27 years old
- Xavier Emmanuel Serrano Rosado, 35 years old
- Christopher Joseph Sanfeliz, 24 years old
- Yilmary Rodriguez Solivan, 24 years old
- Edward Sotomayor Jr., 34 years old
- Shane Evan Tomlinson, 33 years old
- Martin Benitez Torres, 33 years old
- Jonathan Antonio Camuy Vega, 24 years old
- Juan P. Rivera Velazquez, 37 years old
- Luis S. Vielma, 22 years old
- Franky Jimmy Dejesus Velazquez, 50 years old
- Luis Daniel Wilson-Leon, 37 years old
- Jerald Arthur Wright, 31 years old
Fifty-three more people were injured, many of them with life-threatening injuries.